High Performance Thin Layer Chromatographic Method for Estimation of Cefprozil in Tablet Dosage Form

摘要

A simple, fast, specific and precise HPTLC method has been developed for the estimation of cefprozil in pure and in tablet dosage forms. Aluminium plates precoated with silica gel G60F254were used as stationary phase and a mixture of chloroform: methanol: toluene: diethyl amine: water in the ratio 4: 4.4: 3.2: 3: 0.8 v/v as mobile phase. Quantification was carried out by the use of a densitometer in absorbance mode at 286 nm. The Rfvalue of cefprozil was found to be between 0.37 and 0.40. The results of the analysis have been validated statistically and by recovery studies. Linearity was observed in the concentration range of 400-2000 ng/spot.