The functional components and mechanism of Linderae Radix in treating diabetic nephropathy based on the network pharmacology

摘要

This study applied the TCMSP, VBA, OMIM, DiGSeE, TDD, GEO, UniProt, DAVID, STRING a lot of online data and Cytoscape 3 . 2 . 1 analysis software to predict the drug’s active ingredient, and the target of diabetic nephropathy?(DN).?The “drug-target-disease network”?was constructed to analyze the components, targets and pathways of the herb and preliminarily reveal that the treatment of DN?by Wuyao ( Linderae Radix ) is the result of the combined action of multiple components, targets and links, providing a basic research direction for the treatment of DN?by Linderae Radix . O bjective : To analyze the components and mechanisms of?Wuyao ( Linderae Radix )?in treating diabetic nephropathy?(DN)?based on network pharmacology . Methods : Multiple online databases were used to search and screen out the active ingredients?from Linderae Radix , the related targets of active?components of Linderae Radix and the genes related to DN . Search the corresponding genes?name of target through UniProt database . Cytoscape 3 . 2 . 1 was used to construct the corresponding target gene network of Linderae Radix compounds . Venn diagram was used to screen the?intersection genes of the active components corresponding to the target and disease-related genes, and the intersection genes were constructed into the protein interaction relationship network . Finally, DAVID database was used to do GO function enrichment analysis and KEGG signaling pathway enrichment analysis for the intersection genes, and the results of GO and KEGG were visualized . Results : 1 . A total of 7 potential active ingredients and 100 target proteins were screened . 2 . There are a total of 34 intersection genes between the potential active ingredient target in Linderae Radix in DN . 3 . The top 10?of the interaction correlation between intersection proteins include:AR and NCOA2, NCOA2 and NR3C1, NCOA2 and PPARG, etc . 4 . There were 16 entries of molecular function, 9 entries of cell component, 47 entries of biological process and 18 entries of signaling pathway ( P 0.05) . Conclusion : DN was treated by Linderae Radix from multi-component, multi-target and multi-link synergies .