IN VITRO EMBRYO CULTURE OF RARELY ENDANGERED MUSELLA LASIOCARPA (MUSACEAE) WITH EMBRYO DORMANCY

摘要

Musella lasiocarpa (Musaceae) is an ornamental annually producing many viable seeds, but seldomrecruited by seeds in the wild. One mature Musella seed has a small mushroom-shaped embryo withoutdiscernible organ differentiation. Therefore, freshly-harvested mature seeds are dormant. When the seedsgradually finished differentiation during warm stratification at 23°C, they germinated to 82%. Besides,extracted embryos from fresh seeds did not germinate on the basal medium of Murshige and Skoog medium(MS) supplemented with 3% sucrose and 0.8% agar, but they were induced to form calli and root by media. Theoptimum medium for inducing calli was MS + 1.0 mg/L 6-BA + 0.05 mg/L NAA + 100 mg/L Vc with thehighest proliferation coefficient (7.3) in 35 days. Moreover, the embryos from the 6-month warm stratifiedseeds could proliferate on the suitable medium. The optimal medium for rooting was MS + 0.5 mg/L 2, 4-D +Vitamin C 100 mg/L. The results confirmed that both the embryo developmental stage and appropriatecombination of chemicals significantly affected seed germination and In vitro embryo culture of this species.