Neural stem cell conditioned medium alleviates Aβsub25-35/sub damage to SH-SY5Y cells through the PCMT1/MST1 pathway

摘要

Alzheimer’s disease (AD) is a progressive, neurodegenerative disease. Accumulating evidence suggests that protein isoaspartate methyltransferase 1 (PCMT1) is highly expressed in brain tissue (substantia nigra, blue plaque, paraventricular nucleus). In this study, we investigated the effect of neural stem cell conditioned medium alleviates Aβsub25-35/sub damage to SH-SY5Y cells by PCMT1/MST1 pathway. Results demonstrated that Aβsub25-35/sub significantly decreased the cell viability in time and dose dependent manner. However, Neural stem cell-complete medium (NSC-CPM) or NSC-CDM had inhibitory effect on toxicity when fibrillation of Aβsub25-35/sub occurred in their presence and NSC-CDM had a better inhibitor result. An increase of the PCMT1 expression levels was found in Aβsub25-35/sub + NSC-CDM group. sh-PCMT1 significantly reduced the PCMT1, the cell viability and inhibited the protective effect; induced apoptosis and increased the expression of p-MST1. Overexpression of PCMT1 group reversed the effect of Aβsub25-35/sub inhibited the cell viability and Aβsub25-35/sub induced the apoptosis. In conclusion, NSC-CDM corrects the damage of Aβsub25-35/sub to cells by increasing PCMT1, reducing MST phosphorylation.