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Digital PCR - Methods and Protocols

机译:数字PCR - 方法和协议

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Sensitivity, reproducibility, precision and accuracy are recurring words in this book. Indeed, digital PCR (dPCR) represents a major step forward in the quantification of nucleic acids. In this field, for many years it has been used the quantitative PCR (qPCR), a technology that allows a real-time monitoring of DNA amplification which has proved useful for obtaining a relative measurement, but that only indirectly, through the use of reference standards, provides an absolute quantification....
机译:灵敏度,再现性,精度和准确性在本书中是重复的单词。实际上,数字PCR(DPCR)表示核酸的定量前进的重大步骤。在该领域,多年来它已被使用定量PCR(QPCR),这是一种技术,该技术允许对DNA放大的实时监测,这证明了可用于获得相对测量,但仅通过使用参考来间接地进行标准,提供绝对量化....

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