LC-MS study; antioxidant and antidiabetic potential of Morusmacroura Miq. leaves

摘要

Morus leaves are used in folk medicine for hundreds of years as a hypoglycemic. However, leaves of MorusmacrouraMiq. has not yet to be scientifically proven and confirmed their ability to treat diabetes. Objective: The recent workwas aimed to investigate the phytochemical screening of MorusmacrouraMiq. (Moraceae) leaves extracts andevaluate their chemical compositions as well as to overview the antidiabetic activities;total antioxidant capacityviadigestive enzyme and free radical inhibitors which are used to prevent complications resulting from diabetes. Thechemical composition was determined by Liquid Chromatography-Mass Spectrometry (LC-MS).The antioxidantactivity of the extracts and isolated fractions were estimated using 2,2-diphenyl-1-picrylhydrazyl (DPPH),phosphomolybdenum method (PM) assays, while digestive enzymes inhibitory activity was assessed using α-glucosidase inhibitory method as antidiabetic. The phytochemical screening showed that leaves methanol extract of M.macrouraMiq. contain flavonoids, tannins, polyphenols, alkaloids and terpenoids. TheCH 2 Cl 2 and MeOH extractsexhibited antidiabetic activity with IC 50 20.56 and 17.65 μg/mL respectively. LC-MS analysis which showed thepresence of different benzofuran derivatives, Diels–Alder type adducts,phenolic acids, quercetin derivatives andprenylated flavonoids, in which these compounds had high antioxidantand inhibitory activity against α-glucosidase ordiabetes. LC-MS analysis revealed the presence of polyphenolic compoundswhich had high antioxidant and α-glucosidase inhibitory activities, might largely contribute in the antidiabetic activity.