While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease. Kai Mattern, Jakob W. von Trotha, et al. develop NeuroExaminer, an all glass device for whole-brain in vivo imaging in zebrafish. The method is based on light-sheet microscopy and microfluidics and provides insights on brain function in live zebrafish.
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机译：虽然微流体能够具有高时空精度的化学刺激应用，但是光纸显微镜允许具有细胞分辨率的整个斑马鱼大脑的快速成像。然而，这两种技术尚未组合以监测全脑神经活动。与常规的微流体不同，我们在此报告了一种全玻璃装置（神经抑制剂），其与使用光纸显微镜的体内成像中的全脑兼容，因此可以在健康和疾病中提供对脑功能的洞察力。 Kai Mattern，Jakob W.Von Trotha，等。开发神经抑制剂，是斑马鱼类体内成像的全脑的全玻璃装置。该方法基于光纸显微镜和微流体，并为活斑马鱼中的脑功能提供了见解。
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