Crystallization and preliminary X‐ray analysis of the 9 kDa protein of the mouse signal recognition particle and the selenomethionyl‐SRP9

摘要

>Two different crystal forms of the 9 kDa protein of the signal recognition particle (SRP9) have been prepared by the hanging drop vapor diffusion technique using 28% (w/v) PEG8000 or 28% saturated ammonium sulphate as precipitant. The crystals are hexagonal bipyramids with average dimensions of 0.2 × 0.1 × 0.1 mm3 and they diffract to a resolution of 2.3 Å. They belong to the space groups P6₂22/P6₄22 or P3₁21/P3₂21 with cell dimensions data-equation-construct="true" class="math-equation-construct"> data-equation-image="true" class="math-equation-image"> alt="math formula" src="http://onlinelibrary.wiley.com/store/10.1016/0014-5793(96)00316-X/asset/equation/feb2001457939600316x-math-si1.gif?v=1&s=afdbbfa5e844723dee43fd8ce36295600fc85f1d" class="inlineGraphic" /> data-equation-mathml="true" class="math-equation-mathml" style="display:none">. Crystals have also been grown from the selenomethionyl protein and multiwavelength data sets have been collected.