Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen

Fabien Miszczak; Kathleen M. Shuck; Zhengchun Lu; Yun Young Go; Jianqiang Zhang; Stephen Sells

首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen

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Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen

机译：在两个精液中检测马动脉炎病毒的两个TaqMan分析中评估两个基于磁珠的病毒核酸纯化试剂盒和三个实时逆转录PCR试剂系统

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This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.

机译：这项研究表明，在关于核酸提取方法和测试试剂的特定条件下，先前描述的实时逆转录PCR（rRT-PCR）分析（T1分析）提供的敏感性等于或高于病毒分离的敏感性用于检测精液中的马动脉炎病毒。

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《Journal of Clinical Microbiology》 |2011年第10期|共3页
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Fabien Miszczak; Kathleen M. Shuck; Zhengchun Lu; Yun Young Go; Jianqiang Zhang; Stephen Sells;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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专利

1. Detection of equine arteritis virus by real-time TaqMan (R) reverse transcription-PCR assay [J] . Balasuriya UBR, Leutenegger CM, Topol JB, Journal of Virological Methods . 2002,第1a2期

机译：实时TaqMan（R）逆转录PCR检测法检测马动脉炎病毒
2. Detection of equine arteritis virus by real-time TaqMan reverse transcription-PCR assay. [J] . Balasuriya UdeniB R, Leutenegger ChristianM, Topol JB, Journal of Virological Methods . 2002,第1a2期

机译：通过实时TaqMan逆转录PCR检测马动脉炎病毒。
3. Development and evaluation of a reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay for detection of equine arteritis virus in equine semen and tissue samples using the POCKIT (TM) system [J] . Carossino Mariano, Lee Pei-Yu A., Nam Bora, Journal of Virological Methods . 2016,第Null期

机译：使用POCKIT（TM）系统开发和评估用于检测马精液和组织样品中马动脉炎病毒的逆转录绝缘等温聚合酶链反应（RT-iiPCR）分析方法
4. Ultrasensitive digital detection of nanoparticles: Viral diagnostics and multiplexed protein and nucleic acid assays [C] . Unlu M.S. IEEE Photonics Conference . 2014

机译：纳米颗粒的超灵敏数字检测：病毒诊断以及蛋白质和核酸的多重检测
5. Evaluation of the safety of vaccinating mares against equine viral arteritis during mid or late gestation or during the immediate postpartum period and infection of embryos following insemination of donor mares with equine arteritis infective semen. [D] . Broaddus, Charles C. 2011

机译：评估母马在妊娠中期或晚期或产后立即接种疫苗预防马病毒性动脉炎的安全性，以及用马动脉炎感染精液授精供体母猪后对胚胎的感染。
6. Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen [O] . Fabien Miszczak, Kathleen M. Shuck, Zhengchun Lu, 2011

机译：在两个精液中检测马动脉炎病毒的两种TaqMan分析中评估两个基于磁珠的病毒核酸纯化试剂盒和三个实时逆转录PCR试剂系统
7. Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen▿† [O] . Miszczak, Fabien, Shuck, Kathleen M., Lu, Zhengchun, 2011

机译：两种TaqMan测定中两个基于磁珠的病毒核酸纯化试剂盒和三个实时逆转录PCR试剂系统在精液中检测马动脉炎病毒的评估
8. Smallpox and pan-Orthodox Virus Detection by Real-Time 3'-Minor Groove Binder TaqMan Assays Oil the Roche LightCycler and the Cepheid Smart Cycler Platforms [R] . Kulesh, D. A. , Baker, R. O. , Loveless, B. M. , 2003

机译：通过实时3'-次沟槽粘合剂进行天花和泛正统病毒检测Taqman检测为罗氏LightCycler和Cepheid智能循环平台提供油脂

Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen

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