Oxidative stress response in yeast: effect of glutathione on adaptation to hydrogen peroxide stress in Saccharomyces cerevisiae

摘要

>Role of intracellular glutathione in the response of Saccharomyces cerevisiae to H₂O₂ was investigated. Depletion of cellular glutathione or inhibition of γ-glutamylcysteine synthetase (GSH-I) enhanced the sensitivity to H₂O₂ and suppressed the adaptation to H₂O₂. A mutant deficient in GSH-I also showed the hypersensitivity and could not adapt to H₂O₂. Incubation of the cell with amino acids constituting glutathione ( class="smallCaps">l-Glu, class="smallCaps">l-Cys, Gly) increased the intracellular glutathione content, and subsequently the cell acquired resistance against H₂O₂. These results strongly suggest that intracellular glutathione plays an important role in the adaptive response in S. cerevisiae to oxidative damage.