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Oxygen exchange between the Fe(IV)O heme and bulk water for the A2 isozyme of horseradish peroxidase

机译:Fe(IV)O血红素与大量水之间的氧交换作用,用于辣根过氧化物酶的A2同工酶

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>Resonance Raman spectra were observed for compound II of horseradish peroxidase A2, and the Fe(IV)O stretching Raman line was identified at 775 cm−1. This Raman line shifted to 741 cm−1 upon a change of solvent from H2 16O to H2 18O, indicating occurrence of the oxygen exchange between the Fe(IV)O heme and bulk water. The oxygen exchange took place only at the acidic side of the heme-linked ionization with pK a = 6.9.
机译:观察到辣根过氧化物酶A 2 的化合物II的共振拉曼光谱,并在775 cm -1 处鉴定出Fe(IV)O拉伸拉曼谱线。当溶剂从H 2 16 O变为H 2 时,该拉曼线移至741 cm -1 18 O,表明Fe(IV)O血红素与大量水之间发生了氧交换。氧交换仅在血红素连接电离的酸性侧发生,p K a = 6.9。

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