In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus , the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu~(2+)and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu~(2+)on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu~(2+)was 5 mg/L.
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机译：为探讨台湾不动杆菌外植体不定芽再生的最佳培养条件，以无菌苗的茎段为外植体，并采用不同浓度的己二酸二乙基氨基乙基己酸酯（DA-6），激动素（KT），铜将〜（2+）和谷氨酰胺（Gln）分别加入含有6-苄基氨基嘌呤（6-BA）和萘乙酸（NAA）的MS培养基中，记录并分析不定芽的诱导率和诱导倍数。结果表明，在培养基中添加2 mg / L DA-6、0.4 mg / L KT和15 mg / L Gln可以促进不定芽的再生。然而，低浓度可增强Cu〜（2+）对不定芽再生的影响，高浓度可抑制Cu〜（2+）的产生，最佳浓度为5mg / L。
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