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Mechanisms for Photoinactivation of Enterococcus faecalis in Seawater

机译:海水中粪肠球菌的光灭活机理

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Volume 78, no. 22, p. 7776–7785, 2012. Page 7782: Figure 5 is incorrect due to an erroneous negative sign in front of the exponential expressionintheequationcalculatingtherelativeexpressionofsodAandalackofnormalizationbyaninternalcontrolgene.Thecorrect equation for the relative expression ratio (R) is R ? ?E target ?CP target ?control?sample? ? ? ?E ref ?CP ref ?control?sample? ?, where E is the amplification efficiency and CP is the cycle quantification threshold. We recalculated R using the correct equation with the 16S rRNA gene as the referencegene,measuredaccordingtoM.T.Suzuki,L.T.Taylor,andE.F.DeLong(Appl.Environ.Microbiol.66:4605-4614,2000).We found that the revised sodA relative expression ratios are consistent with those reported in our published article. sodA expression increases in E. faecalis exposed to sunlight under oxic conditions but not under anoxic conditions and not in the oxic and anoxic microcosms kept in the dark. The revised relative expression ratios for sodA are 1 at 0 h, 2.5 at 3 h, 1.4 at 4 h, 2.0 at 7 h, and 1.5 at 11 h for the light oxic microcosms, 1 at 0 h, 0.7 at 3 h, 0.8 at 4 h, 0.5 at 7 h, and 0.6 at 11 h for the light anoxic microcosms, 1 at 0 h, 0.8 at 4 h,and0.9at11hforthedarkoxicmicrocosms,and1at0h,0.7at4h,and0.6at11hforthedarkanoxicmicrocosms.Therevisedrelative expression values in the light oxic microcosms are statistically higher than the revised relative expression values in the light anoxic microcosms (paired t test, P ? 0.04). As stated in the article, these results suggest that E. faecalis in the light oxic microcosms is responding to photooxidative damage. Thus, the conclusions in the original article are unchanged.
机译:第78卷,没有。 22页7776–7785,2012年。Page 7782:图5是不正确的,这是由于在计算内部控制基因的sodAandalack归一化的等式的等式时,指数表达式前面的负负号是错误的。相对表达比(R)的正确方程为R? “ E目标”“ CP目标”“对照”“样品” ? ? ?E ref?CP ref?对照?样品? α,其中E是放大效率,CP是循环定量阈值。我们以16S rRNA基因为参考基因,使用正确的方程式重新计算R,并根据铃木M.T.铃木,LTTaylor和E.F.德隆(Appl.Environ.Microbiol.66:4605-4614,2000)进行了测量。修改后的sodA相对表达率与我们发表的文章中报道的一致。 sodA表达在有氧条件下暴露于阳光的粪便中增加,但在无氧条件下则没有,并且在黑暗中保持的有氧和无氧微观世界中也没有。 sodA的修订相对表达率对于轻度有氧微观世界是0小时1、3小时2.5、4 4小时1.4、2.0 7小时和11小时1.5、0小时1、3小时0.7、0.8轻度缺氧的微观世界在4 h,0.5 7时和11 h在0.6时,0 h时1在4 h时,0.8在4 h时,三氧化二砷为0.9at11h,darkanoxic微观地为1at0h,0.7 at4h和0.6 at 11 h。微观缺氧统计学上高于轻度缺氧微观校正的相对表达值(配对t检验,P≤0.04)。如文章中所述,这些结果表明,轻氧化微观世界中的粪肠球菌对光氧化损伤有反应。因此,原始文章中的结论不变。

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