Transcriptome Analysis of an Antibiotic Downregulator Mutant and Synergistic Actinorhodin Stimulation via Disruption of a Precursor Flux Regulator in Streptomyces coelicolor

摘要

Through microarray analysis of an antibiotic-downregulator-deleted Streptomyces coelicolor Δ wblA ΔSCO1712 mutant, 28 wblA - and SCO1712-dependent genes were identified and characterized. Among 14 wblA - and SCO1712-independent genes, a carbon flux regulating 6-phosphofructokinase SCO5426 was additionally disrupted in the Δ wblA ΔSCO1712 mutant and further stimulated actinorhodin production in S. coelicolor , implying that both regulatory and precursor flux pathways could be synergistically optimized for antibiotic production.