Aromatic Hydroxylation of Indan by o-Xylene-Degrading Rhodococcus sp. Strain DK17

摘要

The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o -xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o -xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis -indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-Indandiol undergoes ring cleavage by a meta -cleavage dioxygenase.