Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial

M. H. Josefsen; N. Cook; M. DAgostino; F. Hansen; M. Wagner; K. Demnerova; A. E. Heuvelink; P. T. Tassios; H. Lindmark; V. Kmet; M. Barbanera; P. Fach; S. Loncarevic; J. Hoorfar

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Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial

机译：在多中心协作试验中检测基于食物的耐高温弯曲杆菌的基于PCR的方法的验证

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A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.

机译：通过与12个实验室一起测试加标car体冲洗样品的合作试验，评估了一种基于PCR的快速检测食源性耐热弯曲杆菌的方法。该方法对鸡样品的实验室间诊断灵敏度为96.7％，诊断特异性为100％，而对于猪样品，这些值分别为94.2和83.3％。

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《Applied and Environmental Microbiology》 |2004年第7期|共5页
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M. H. Josefsen; N. Cook; M. DAgostino; F. Hansen; M. Wagner; K. Demnerova; A. E. Heuvelink; P. T. Tassios; H. Lindmark; V. Kmet; M. Barbanera; P. Fach; S. Loncarevic; J. Hoorfar;
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1. Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial [J] . M. H. Josefsen, N. Cook, M. DAgostino, Applied and Environmental Microbiology . 2004,第7期

机译：在多中心协作试验中检测基于食物的耐高温弯曲杆菌的基于PCR的方法的验证
2. Toward an International Standard for PCR-Based Detection of Food-Borne Thermotolerant Campylobacters: Validation in a Multicenter Collaborative Trial [J] . P. S. Lübeck, N. Cook, M. Wagner, Applied and Environmental Microbiology . 2003,第9期

机译：迈向基于PCR的食品耐高温弯曲杆菌检测国际标准：在多中心协作试验中的验证
3. Toward an International Standard for PCR-Based Detection of Food-Borne Thermotolerant Campylobacters: Assay Development and Analytical Validation [J] . P.S. Lübeck, P. Wolffs, S. L. W. On, Applied and Environmental Microbiology . 2003,第9期

机译：迈向基于PCR的食品耐高温弯曲杆菌细菌检测国际标准：分析开发和分析验证
4. Multicenter clinical trials of imaging workstations and methods [C] . C.R. Welsh, V. Gopalakrishnan, J. Flaim-Spetsas, Internationao Congress and Exhibition . 2002

机译：成像工作站和方法的多中心临床试验
5. Validation of an improved isolation and detection method for Campylobacter jejuni in various foods [D] . Odongo, Rose 2007

机译：改良的空肠弯曲菌在各种食品中的分离和检测方法的验证
6. Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial [O] . M. H. Josefsen, N. Cook, M. DAgostino, 2004

机译：在多中心协作试验中检测基于食物的耐高温弯曲杆菌的基于PCR的方法的验证
7. Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial [O] . Josefsen, M. H., Cook, N., D'Agostino, M., 2004

机译：在多中心协作试验中检测基于食物的耐高温弯曲杆菌的基于PCR的方法的验证
8. Collaborative Multicenter Trial of Rimantadine [R] . Betts, R. F., Dolin, R., Graman, P. S., 1990

机译：金刚乙胺的协同多中心试验

Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial

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