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首页> 外文期刊>Applied and Environmental Microbiology >Mechanism of action of benzoic acid on Zygosaccharomyces bailii: effects on glycolytic metabolite levels, energy production, and intracellular pH.
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Mechanism of action of benzoic acid on Zygosaccharomyces bailii: effects on glycolytic metabolite levels, energy production, and intracellular pH.

机译:苯甲酸对百日咳酵母的作用机理:对糖酵解代谢产物水平,能量产生和细胞内pH的影响。

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The effects of benzoic acid in the preservative-resistant yeast Zygosaccharomyces bailii were studied. At concentrations of benzoic acid up to 4 mM, fermentation was stimulated and only low levels of benzoate were accumulated. Near the MIC (10 mM), fermentation was inhibited, ATP levels declined, and benzoate was accumulated to relatively higher levels. Intracellular pH was reduced but not greatly. Changes in the levels of metabolites at different external benzoic acid levels showed that glycolysis was limited at pyruvate kinase and glyceraldehyde dehydrogenase-phosphoglycerate kinase steps. Inhibition of phosphofructokinase and several other glycolytic enzymes was not responsible for the inhibition of fermentation. Instead, the results suggest that the primary action of benzoic acid in Z. bailii is to cause a general energy loss, i.e., ATP depletion.
机译:研究了苯甲酸在耐防腐剂酵母百日酵母中的作用。在高达4 mM的苯甲酸浓度下,刺激了发酵,仅积累了少量的苯甲酸酯。在MIC(10 mM)附近,发酵受到抑制,ATP含量下降,苯甲酸盐积累到相对较高的水平。细胞内pH降低但不大大降低。不同外部苯甲酸水平下代谢物水平的变化表明,糖酵解作用仅限于丙酮酸激酶和甘油醛脱氢酶-磷酸甘油酸激酶步骤。磷酸果糖激酶和其他几种糖酵解酶的抑制作用与抑制发酵无关。取而代之的是,结果表明百日咳杆菌中苯甲酸的主要作用是引起一般的能量损失,即ATP耗竭。

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