Photophysical Study of 2‐Fluorenyl‐1,2,3‐Triazole‐labeled 2′‐Deoxyuridine and Its Oligonucleotide

摘要

In this study, we prepared 2‐fluorenyl‐1,2,3‐triazol‐4‐yl‐2′‐deoxyuridine (U FT) and inserted it at the central position of an oligodeoxynucleotide (ODN) to investigate whether the triazole group is an efficient linker between fluorene and uracil. U FT displays different fluorescence intensities and emission maxima in different solvents, indicating its potential for application as an environmentally sensitive probe. Moreover, excimer emission from U FT was observed in water. However, U FT containing ODN did not exhibit selective thermal stability and fluorescence changes upon duplex formation with matched and single‐base mismatched targets. This highlighted the importance of selecting the appropriate linker to connect the fluorophore to the nucleobase when developing such DNA probes.