Antioxidative properties of the leaves of Daphniphyllum chartaceum Rosenthal

摘要

In vitro antioxidative properties of methanol, chloroform and diethyl ether extracts of the leaves of?Daphniphyllum chartaceum?Rosenthal. were examined spectrophotometrically.?In vitro?antioxidant activity of all the extracts were determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay, reducing power assay, hydrogen peroxide (H2O2)?scavenging activity and total phenol content. Among all the three extracts, diethyl ether showed highest DPPH scavenging (32.78±0.11) at a concentration of 1000 μg/ml and reducing power activity (0.908±0.013). The methanolic extract exhibited higher phenolic content (0.19±0.013 mg/ml catechol equivalent) and as a result, there was an enhanced H2O2?scavenging activity (27.93±0.47), followed by the chloroform extract. The observations from this study suggest the potentiality of?D.?chartaceum, which could be commercially exploited by the pharmaceutical industry as a natural antioxidant.