In-vivo Oxidative DNA damage, Protein Oxidation and Lipid Peroxidation as a Biomarker of Oxidative stress in Preterm Low Birth Weight Infants

摘要

Pregnancy while not a disease is often accompanied by a high energy demand of many bodily functions. Neonates are said to be more susceptible to oxygen radical injury. The objective of the present study was to measure cord blood levels of 8-OHdG, malondialdehyde, protein carbonyl and total antioxidant status in premature low birth weight infants to analyze the status of oxidative stress in relation to the degree of prematurity and birth weight of neonates. Umbilical cord blood samples were obtained at the time of delivery. 8-OHdG has been measured as oxidative DNA damage marker in preterm LBW newborns by competitive in vitro Enzyme-Linked Immunosorbent Assay (ELISA) along with malondialdehyde as marker of lipid peroxidation , protein carbonyl as marker of protein oxidation and total antioxidant status to study the oxidative stress . Significant elevation in the levels of 8-OHdG along with malondialdehyde, protein carbonyl has been noted in preterm LBW newborns. Serum 8-OHdG is found to be significantly and negatively correlated with birth weight (r = -0.834, p<0.001) and gestational age of the newborn (r = -0.626, p<0.001). These results provide evidence of increased oxidative stress in the form of DNA damage, protein oxidation and lipid peroxidation in premature LBW newborns which may be responsible for different complications associated with prematurity.