Effect of Xiaoaiping combined with 5-fluorouracil treatment on liver cancer cell growth in vitro and oncogene expression

摘要

Objective: To study the effect of Xiaoaiping combined with 5-fluorouracil treatment on livercancer cell growth in vitro and oncogene expression. Methods: Liver cancer cell lines SMMC-7721 were cultured and treated with different doses of Xiaoaiping (10, 20 and 40 μL/mL) and5-fluorouracil (10, 20 and 40 μmol/mL). 12 h, 24 h and 48 h after treatment, the CCK-8 kitswere used to determine the cell viability; 24 h after treatment, enzyme-linked immunosorbentassay kits were used to determine proliferation and apoptosis gene expression. Results: 12 h,24 h and 48 h after treatment, different doses of Xiaoaiping and 5-fluorouracil could all reducecell proliferation activity in dose-dependent manner and the cell proliferation activity of 40 μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group were significantly lower than those of 40 μL/mLXiaoaiping group and 40 μmol/mL 5-Fu group; 24 h after treatment, P16INK4, P57kip2, Caspase-3and PDCD5 protein expression in 40 μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group, 40 μL/mLXiaoaiping group and 40 μmol/mL 5-Fu group were significantly higher than those in controlgroup while LETM1, SIRT6 and UHRF1 protein expression were significantly lower thanthose in control group, and P16INK4, P57kip2, Caspase-3 and PDCD5 protein expression in 40μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group were significantly higher than those in 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group while LETM1, SIRT6 and UHRF1 proteinexpression were significantly lower than those in 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group. Conclusion: Xiaoaiping combined with 5-fluorouracil has inhibitory effecton liver cancer cell proliferation, and can also increase the expression of apoptosis genes andreduce the expression of proliferation genes.