Correlation of peripheral blood mir-146b and mir-155 expression with Treg immunity and myocardial injury in children with viral myocarditis

摘要

Objective: To study the correlation of peripheral blood mir-146b and mir-155 expressionwith Treg immunity and myocardial injury in children with viral myocarditis. Methods: Thechildren who were diagnosed with viral myocarditis in Wuhan Children’s Hospital betweenJune 2014 and February 2017 were selected as the VMC group of the study, and the childrenwho received physical examination during the same period were selected as the control groupof the study. Peripheral blood was collected to determine the expression of mir-146b, mir-155and immune transcription factors, and the serum was collected to determine the contents ofimmune cytokines and myocardial injury indexes. Results: Peripheral blood mir-146b and mir-155 expression of VMC group were significantly higher than those of control group; peripheralblood Foxp3 mRNA expression as well as serum IL-10 and TGF-β contents of VMC groupwas significantly lower than those of control group and negatively correlated with mir-146band mir-155 expression while peripheral blood ROR-γt mRNA expression as well as serumIL-17, IL-21, CK-MB, cTnI, GrB, sFasL and caspase-3 contents was significantly higherthan those of control group and positively correlated with mir-146b and mir-155 expression.Conclusion: Highly expressed mir-146b and mir-155 in peripheral blood of children with viralmyocarditis can inhibit Treg immunity and increase myocardial injury.