Correlation of Claudins6 (CLDN6) gene expression in meningioma tissue with the expression of matrix metalloproteinases (MMPs)/tissue inhibitors of matrix metalloproteinase (TIMPs) and epithelial-mesenchymal transition (EMT) genes

摘要

Objective: To study the correlation of Claudins6 (CLDN6) gene expression in meningiomatissue with the expression of matrix metalloproteinases (MMPs)/tissue inhibitors of matrixmetalloproteinase (TIMPs) and epithelial-mesenchymal transition (EMT) genes. Methods:Meningioma tissue samples that were surgically removed in Yibin First People’s Hospitalbetween April 2014 and May 2017 were selected, normal arachnoid tissue samples thatwere collected from decompressive craniectomy in Yibin First People’s Hospital during thesame period were selected, and the expression of CLDN6, MMPs/TIMPs and EMT genesin tissues were determined. Results: CLDN6 protein expression in meningioma tissuewas significantly lower than that in normal arachnoid tissue; EMMPRIN, MMP2, MMP9,Vimentin and N-cadherin protein expression in meningioma tissue were significantly higherthan those in normal arachnoid tissue while TIMP1, TIMP2, E-cadherin and α-cateninprotein expression were significantly lower than those in normal arachnoid tissue; EMMPRIN,MMP2, MMP9, Vimentin and N-cadherin protein expression in meningioma tissue withhigher CLDN6 expression were significantly lower than those in meningioma tissue withlower CLDN6 expression while TIMP1, TIMP2, E-cadherin and α-catenin protein expressionwere significantly higher than those in meningioma tissue with lower CLDN6 expression.Conclusion: Lowly expressed CLDN6 gene in meningioma tissue can increase the hydrolysisactivity of MMPs, induce epithelial-mesenchymal transition and thus promote the invasivegrowth of meningioma.