Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots

摘要

An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of?Korean wild ginseng ( Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the?ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5?mg/L 2,4-dichlorophenoxyacetic acid and 0.3?mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5?mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5?mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5?mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25?mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. Keywords: mutant adventitious roots, Panax ginseng Meyer, somatic embryo, somatic embryogenesis.