Protective effect of cardioplegia with poly (ADP-ribose) polymerase-1 inhibitor against myocardial ischemia-reperfusion injury: in vitro study of isolated rat heart model

摘要

Abstract Poly(ADP-ribose) polymerase (PARP)-1 inhibitor has been suggested to attenuate the ischemia-reperfusion injury. We investigated the protective effect of the cardioplegia with a PARP-1 inhibitor, 4-hydoxyquinazoline (4-HQ), against myocardial ischemia-reperfusion injury. Isolated rat hearts were perfused on a Langendorff apparatus and cardioplegically arrested for 90?min by perfusion with St. Thomas’ Hospital solution (ST-solution). In the Group ST (n?=?8), the hearts were arrested with the ST-solution alone. The Group HQ (n?=?8) were treated with the ST-solution containing 4-HQ (10 μM) for cardioplegia. During reperfusion, the Group HQ showed significantly greater functional recovery of +dp/dt_max (p?=?0.005) and lower enzymatic leakage (p?+ levels were also preserved higher in the Group HQ (p?+ consumption and suppressing oxidative stress.