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Analysis of short fruiting branch gene and Marker-assisted selection with SNP linked to its trait in upland cotton

机译:陆地棉短果枝基因分析及SNP与其性状相关的标记辅助选择

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With the rapid development of genomics, many functional genes have been targeted. Molecular marker assisted selection can accelerate the breeding process by linking selection to functional genes. In a study of upland cotton (Gossypium hirsutum L.), the F2 segregated population was constructed by crossing X1570 (short branches) with Ekangmian-13 (long branches) to identify the short fruiting branch gene and marker-assisted selection with SNP(Single Nucleotide Polymorphisms, SNP) linked to its trait. The result demonstrated that linked SSR marker BNL3232 was screened by BSA(Bulked segregant analysis, BSA) method; one SNP locus was found, which was totally separated from the fruiting branches trait in upland cotton. It was verified that this SNP marker could be used for molecular assisted selection of cotton architecture.
机译:随着基因组学的快速发展,许多功能基因已经成为目标。分子标记辅助选择可以通过将选择链接到功能基因来加速育种过程。在一项陆地棉研究中,通过将X1570(短枝)与Ekangmian-13(长枝)杂交来鉴定F2隔离种群,以鉴定短结果枝基因并用SNP(Single)进行标记辅助选择核苷酸多态性(SNP)与其性状有关。结果表明,采用BSA(Bulked segregant analysis,BSA)方法筛选了SSR标记BNL3232。发现了一个SNP基因座,它与陆地棉的果枝性状完全分离。证实了该SNP标记可用于分子辅助选择棉花结构。

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