Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry

摘要

This paper was conducted to develop a method containing ultrahigh performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for simultaneous quantification of six bioactive components in the decoction of Ziziphi spinosae Semen. Analysis was performed on an Agilent ZORBAX Extend-C18 column (2.1?×?100?mm, 1.8?μm) and eluted with a mobile phase system consisting of acetonitrile and water under a gradient program with a flow rate of 0.3?ml/min. The injection volume was 2?μl. Multiple-reaction monitoring scanning detection was employed for quantification with an electrospray ion source in the negative ion mode. All the six compounds showed good linearities (). The LODs of the six bioactive compounds were 0.039?ng/ml, 0.092?ng/ml, 3.112?ng/ml, 2.131?ng/ml, 0.099?ng/ml, and 0.071?ng/ml for spinosin, 6?-feruloylspinosin, jujuboside A, jujuboside B, camelliaside B, and betulinic acid, respectively. The LOQs were 0.118?ng/ml, 0.276?ng/ml, 9.336?ng/ml, 6.393?ng/ml, 0.299?ng/ml, and 0.213?ng/ml for spinosin, 6?-feruloylspinosin, jujuboside A, jujuboside B, camelliaside B, and betulinic acid, respectively. According to our knowledge, it was the first time to establish a method with high efficiency and accuracy for the quantification of six bioactive components in the decoction of Ziziphi spinosae Semen, which would provide references for quality control and evaluation of Ziziphi spinosae Semen.