Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3βand APC and Inhibiting Geneβ-Catenin

摘要

Objective.Inhibiting geneβ-catenin and inducting genes GSK-3βand APC, promoting the tumor cell apoptosis in Wnt pathway, by chlorogenic acid were discussed (CGA).Method.The different genes were scanned by the 4*44K mouse microarray chips. The effect of the three genes was confirmed by RT-PCR technique with CGA dosage of 5, 10, and 20 mg/kg.Result.The expression of GSK-3βand APC was upregulated in group of 20 mg/kg dosage (P<0.05) and the expression ofβ-catenin was downregulated in the same dosage (P<0.05).Conclusion.The results infer that the multimeric protein complex ofβ-catenin could be increased by CGA upregulated genes GSK-3βand APC, which could inhibit the freeβ-catenin into the nucleus to connect with TCF. So the transcriptional expression of the target genes will be cut to abnormal cell proliferation. It is probably one of the ways that can stop the tumor increase by CGA.