Effect of Gypsum fibrosum and Rhizoma atractylodis on In Vitro Proliferation of Mouse Splenic Lymphocyte

摘要

Qingliang electuary plays important roles in speeding up heat dissipation, enhancing blood circulation and improving the body?s immune system. The main ingredients of a qingliang electuary are Gypsum fibrosum (GF) and Rhizoma atractylodis (RA). Here, researchers report the effect of these two ingredients on in vitro proliferation of mouse splenic lymphocyte. The extracts of GF and RA along with ConA were tested under high temperatures (37, 39 and 42°C) in combination with different incubation times (1 or 4 h) using the MTT (3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide) Method. The results showed that ConA significantly stimulated lymphocyte proliferation at 37°C. Under 39 and 42°C, the stimulation diminished as incubation time prolonged. High dose GF (500 μg mL-1) significantly inhibited proliferation under all tested conditions but low dose GF (0.05 μg mL-1) significantly increased lymphocyte proliferation at 42°C after 1 h incubation. RA water extract at 500 μg mL-1 significantly increased lymphocyte proliferation under all tested conditions. The effect of RA oil was weaker compared to RA water extract and not consistent under different incubation conditions.