...
首页> 外文期刊>Journal of animal and veterinary advances >Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (Ovis aries) Genes SLC39A1, SLC39A2 and SLC39A7
【24h】

Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (Ovis aries) Genes SLC39A1, SLC39A2 and SLC39A7

机译:三种绵羊(绵羊)新基因SLC39A1,SLC39A2和SLC39A7的分子克隆,序列鉴定和组织表达谱

获取原文
           

摘要

The complete coding sequences of three sheep genes SLC39A1, SLC39A2 and SLC39A7 were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Sequence analysis revealed that sheep SLC39A1 gene encodes a protein of 324 amino acids that shares high homology with the solute carrier family 39 (zinc transporter), member1 (SLC39A1) proteins of eleven species-goat (99%), cattle (99%), pig (95%), horse (94%), rhesus monkey (94%), chimpanzee (94%), human (94%), rabbit (94%), crab-eating macaque (93%), mouse (93%) and rat (92%). The sheep SLC39A2 gene encodes a protein of 309 amino acids that shares high homology with the solute carrier family 39 (zinc transporter), member 2 (SLC39A2) proteins of eleven species-goat (98%), cattle (95%), horse (81%), giant panda (80%), human (79%), rhesus monkey (78%), chimpanzee (78%), rabbit (77%), Northern white-cheeked gibbon (77%), mouse (75%) and rat (74%). The sheep SLC39A7 gene encodes a protein of 469 amino acids that shares high homology with the solute carrier family 39 (zinc transporter), member 7(SLC39A7) proteins of thirteen species-cattle (98%), dog (93%), pig (94%), chimpanzee (93%), human (93%), horse (93%), rat (93%), rhesus monkey (93%), white-tufted-ear marmoset (92%), Northern white-cheeked gibbon (92%), sumatran orangutan (92%), rabbit (89%) and mouse (86%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302552, 100302553 and 100302555. The phylogenetic analysis revealed that the sheep SLC39A1 and SLC39A2 genes both have closer genetic relationships with the SLC39A1 and SLC39A2 genes of goat. The sheep SLC39A7 gene has a closer genetic relationship with the SLC39A7 gene of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep SLC39A1, SLC39A2 and SLC39A7 genes were all generally but differentially expressed in detected tissues.
机译:使用逆转录聚合酶链反应(RT-PCR)扩增了三个绵羊基因SLC39A1,SLC39A2和SLC39A7的完整编码序列。序列分析显示,绵羊SLC39A1基因编码324个氨基酸的蛋白质,与11种山羊(99%),牛(99%)的溶质载体家族39(锌转运蛋白),member1(SLC39A1)蛋白具有高度同源性,猪(95%),马(94%),恒河猴(94%),黑猩猩(94%),人(94%),兔(94%),食蟹猕猴(93%),小鼠(93%) )和大鼠(92%)。绵羊SLC39A2基因编码的309个氨基酸的蛋白质与溶质载体家族39(锌转运蛋白),11种山羊(98%),牛(95%),马( 81%),大熊猫(80%),人(79%),恒河猴(78%),黑猩猩(78%),兔子(77%),北部白颊长臂猿(77%),小鼠(75%) )和大鼠(74%)。绵羊SLC39A7基因编码469个氨基酸的蛋白质,与溶质载体家族39(锌转运蛋白),13种牛(98%),狗(93%),猪(93%)的成员7(SLC39A7)蛋白具有高度同源性。 94%),黑猩猩(93%),人(93%),马(93%),大鼠(93%),恒河猴(93%),白簇耳耳mo猴(92%),北白颊长臂猿(92%),苏门答腊猩猩(92%),兔子(89%)和小鼠(86%)。最后,将这三个新的绵羊基因分配给了GeneID:100302552、100302553和100302555。系统发育分析表明,绵羊SLC39A1和SLC39A2基因均与山羊的SLC39A1和SLC39A2基因具有更紧密的遗传关系。绵羊SLC39A7基因与牛的SLC39A7基因有更紧密的遗传关系。还进行了组织表达谱分析,结果表明绵羊SLC39A1,SLC39A2和SLC39A7基因在检测到的组织中均普遍表达但差异表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号