Assessment of Ketamine/Dexmedetomidine Anesthesia in Renal Ischemia-Reperfusion Injury in Nude Rats

摘要

In order to perform surgical manipulations for model development an appropriate anesthetic protocol is important to avoid mortality and morbidity that negatively impact a study. Reversible injectable anesthetics are favored in comparison to non-reversible anesthetics because of the presumed safety. Rats responses to injectable anesthetics are determined by the drug, route and individual differences. The group studies kidney regeneration but researchers must first surgically produce a model of chronic renal failure which requires anesthesia. In this study, researchers report the mortality results of using four different anesthetic protocols, Ketamine-Dexmedetomidine (KD), Intramuscularly (IM) with ketoprofen and Buprenorphine (BP); KD-Intraperitoneally (IP) with ketoprofen and buprenorphine; KD and BP IM and Pentobarbital (PB) and BP, IP. Male Nude Rats (250-350 g; n = 40) were anesthetized with anesthetic regiments noted above. After a laparotomy, a bilateral 60 min renal ischemia followed by reperfusion procedure was done. The animals were given antipamezole, a reversal agent when appropriate. PB with BP, IP significantly improved mortality rates at 20%. This study shows that kidneys exposed to ischemia/reperfusion result in renal tissue damage as well as decreased renal function. The results showed increased mortality using ketamine and significantly with favorable survivability using pentobarbital. Future, studies are needed to determine how pentobarbital provided mortality protection.