Optimization of Extraction Procedure for Chinese Truffle Tuber sinense Polysaccharides and Effect of the Polysaccharides on Phagocytosis of Macrophage in vivo

摘要

In this study, Response Surface Methodology (RSM) was employed to optimize the extraction conditions (extraction temperature, ratio of water to raw material and extraction time) of polysaccharides from the fruiting bodies of Chinese truffle Tuber sinense. A Box-Behnken design was used for experimental design and analysis of the results to obtain the optimal processing parameters. The optimal conditions were extraction temperature 94.75°C, ratio of water to raw material 16.41:1, extraction time 2.67 h. According to analysis, extraction temperature was the most significant factor to affect the yield of polysaccharides from Chinese truffle Tuber sinense. Pharmacological experiments indicated that CTP had potent immunomodulatory effects in immunosuppression mice model. At the dose of 50, 100 and 200 mg kg-1 body weight, a significant increase (p<0.01) in phagocytosis of macrophage was observed.