Astragalus polysaccharides induced gene expression profiling of intraepithelial lymphocytes in immune-suppressed mice

摘要

Astragalus polysaccharides (APS) possess a variety of immunomodulatory activities, but the regulation on mucosal immunity is not fully understood. In this study, immune suppression in mice was induced with cyclophosphamide treatment and APS was used as an intervention and was administrated at dosages of 3 g/kg (APS HD) and 1.5 g/kg (APS LD). Intraepithelial lymphocytes (IELs) were isolated from the intestines. The mRNA expressions from IELs in different groups of mice were detected using gene expression microarray to explore the gene expression profile of IELs, and what are the unique pathways related to low and high dosage of APS.