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Transcriptional Changes in Cancer Cells Induced by Exposure to a Healing Method

机译:暴露于治疗方法引起的癌细胞转录变化

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Energy healing, or healing with intent, is a complementary and alternative medicine therapy reported to be beneficial with a wide variety of conditions. We are developing a delivery technology for a method previously tested in mouse models with solid tumors (the Bengston method) independent of the presence of a healer. The goal of this study was to assess whether stored or recorded energy has an impact on breast cancer cells in vitro, using energy-charged cotton and electromagnetic recording of healers practicing the method. Expression of genes involved in cancer and inflammation pathways was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Treatment of cells using energy-charged cotton resulted in statistically significant changes 1.5-fold change when compared to the control, and 68 genes showing statistically significant fold changes. Two genes, ATP citrate lyase (ACLY) and interleukin 1β (IL-1β), were consistently downregulated at 4 and 24 hours of exposure to the recording, respectively, in 3 independent experiments. Both ACLY and IL-1β were also downregulated in cells exposed to a hands-on delivery of the method, suggesting these 2 genes as potential markers of the healing method.
机译:能量治愈或意图治愈是据报道对多种疾病有益的补充和替代药物疗法。我们正在为先前在实体瘤小鼠模型中测试过的方法(Bengston方法)开发一种治疗技术,该方法与治疗剂的存在无关。这项研究的目的是使用能量充足的棉花和实践方法的治疗师的电磁记录来评估储存或记录的能量在体外是否对乳腺癌细胞产生影响。通过定量逆转录聚合酶链反应(qRT-PCR)来测量参与癌症和炎症途径的基因的表达。与对照相比,使用带能量的棉花处理细胞导致统计学上的显着变化,即变化为对照的1.5倍,并且68个基因显示了统计学上的显着倍数变化。在3个独立的实验中,分别在暴露于录音的4小时和24小时,ATP基因柠檬酸裂合酶(ACLY)和白介素1β(IL-1β)一直被下调。在手动操作该方法的细胞中,ACLY和IL-1β均下调,表明这两个基因可能是该治疗方法的潜在标志物。

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