SREBP-2 1784G/CGenotype is Associated with Non-Alcoholic Fatty Liver Disease in North Indians

摘要

Background:Genetics of non-alcoholic fatty liver (NAFLD) in Asian Indians has been inadequately investigated. This study aims to determine the association of the1784G > Cpolymorphism in the SREBP-2 gene with NAFLD in Asian Indians in north India.Methods:In this study, (n= 335); 162 obese with NAFLD, 91 obese without NAFLD and 82 non-obese without NAFLD subjects were recruited. Abdominal ultrasound, clinical profile, anthropometry, metabolic profile, serum levels of alanine aminotransferase, aspartate aminotransferase, fasting insulin and high sensitivity C-reactive protein (hs-CRP) were analysed. Polymerase chain reaction and restriction fragment length polymorphism were used to identify individual genotypes, and the association of this polymorphism with clinical and biochemical parameters was assessed.Results:The observed frequency of G allele was 0.73 and C allele was 0.27. Frequency ofC/Cgenotype was higher in NAFLD as compared to obese and non-obese subjects (p= 0.003). In NAFLD subjects 57.4% wereG/Ghomozygous, 31.5%G/Cheterozygous and 11.1% wereC/Chomozygous. The SREBP-2 genotype frequencies deviated from the Hardy Weinberg Equilibrium (X2= 6.39,p = 0.0114). Mean values of TG (p= 0.002), TC (p= 0.002), ALT (p= 0.04) and AST (p=0.03) levels were significantly higher in NAFLD subjects withG/Cgenotype as compared toG/Ggenotypes in obese and non-obese groups. Fasting insulin (p= 0.03), HOMA (p= 0.009) and hs-CRP levels were significantly higher in NAFLD subjects withG/Cgenotype as compared to obese and non obese subjects withG/Ggenotypes.Conclusion:In this study, conducted for the first time in Asian Indians,SREBP-21784G > Cgenotype was associated with NAFLD.