R.T.R? promotes bone marrow mesenchymal stem cells osteogenic differentiation by upregulating BMPs/SMAD induced cbfa1 expression

摘要

Bone marrow mesenchymal stem cells (BMSCs) of rats were isolated and Bio-Oss~(?) and R.T.R~(?) materials were used in this study.Alkaline phosphatase (ALP) activity in each group was calculated. The ability of adhesion and proliferation of BMSCs on Bio-Oss~(?) and R.T.R~(?) increased with time, but they were significantly higher on R.T.R~(?) than that of Bio-Oss~(?) at all time points (p <0.05). Compared with Bio-Oss~(?), R.T.R~(?) could promote the expression and activity of ALP in BMSCs, and the expression of bone formation related transcription factors bone morphogenetic protein-1 (BMP-1), Cbfa1 and osteoblast marker genes ALP , collagen I , osteopontin , osseomucin and osteocalcin . The expression levels of cbfa1 , ALP , collagen I , osteopontin , osseomucin and osteocalcin were inhibited after downregulated expression of BMP-1 in BMSCs and inoculation with R.T.R~(?). R.T.R~(?) could up-regulate BMP-1 expression and cbfa1 expression through BMPs/SMAD signaling pathway, thereby promoting the expression of ALP , collagen I , osteopontin , osseomucin and osteocalcin and promoting osteoblast differentiation.