A Novel Photoshop-Based Digital Imaging Method for Measuring ColonyExtension of Biocontrol Fungi

摘要

A cost-effective, simple and easy to use method was developed using scanned computer images and softwareprograms to measure the colony extension area of biocontrol fungi on Petri dish. Data were collected daily and archivedorderly onto a model sheet. This sheet was scanned and saved as JPEG form. Tracing areas in JPEG image were measureddirectly based on the integrated reference, using “Magic Wand tool” and “Histogram” in the Photoshop. Formula for calculatingthe pixel data was described. Low coefficient of variation and stable deviation rate were found. Therefore, thismethod would be of suitable and easy to handle for basic colony extension research.