Enzymatic transesterification by using lipase as catalyst has become more attractive for the production of biodieseldue to easy separation of products such as glycerol and fatty acid esters. Comparative studies of enzyme catalyzedtransesterification of three less attended non edible oils (jatropha, karanja and putranjiva) have been discussed. Themaximum yield has been obtained at 3:1 molar ratio of methanol and oil at 40°C and 8hrs reaction time. The percentageof conversion has been analyzed by Gas chromatography and 400 MHz 1H NMR. It has been observed that degummedvegetable oil gives higher conversion in transesterification reaction than straight vegetable oil. Secondary alcohol givesbetter result than methanol for the production of biodiesel due to its higher miscibility nature in oil.
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