An Animal Model for the Study of Lung Protective Therapies During One Lung Ventilation (OLV) in Children

摘要

One-lung ventilation (OLV) is the intentional collapse of one lung during surgical procedures in the chest. Theobjective of this report is to demonstrate that a piglet model of pediatric OLV mimics the clinical sequelae, and thus isvalid to study lung protective interventions. Pediatric pigs (n = 7; 21 ± 1 d old; 7.7 ± 0.5 kg) were instrumented and exposedto OLV (left lung collapsed) for 3 hrs, followed by 30 min of bilateral lung ventilation (BLV) before sacrifice andtissue harvest. Pulmonary compliance (Cp) and resistance (Rp) were measured half-hourly and plasma samples weredrawn hourly for inflammatory cytokine analysis. Cp dropped to 86% and Rp rose to 148% of baseline with OLV, whichis consistent with clinical observations given the anatomical differences of the pig thorax. Plasma levels of TNF- and IL-6 rose throughout the protocol, consistent with clinical observations. Lung histology showed greater atelectasis in the collapsedlungs following 30 min of BLV. The piglet model is valid for the study of OLV-induced lung injury and may beuseful for the study of potential therapies to improve outcomes in the pediatric population.