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Variations in α–, β–Amylase and α–Glycosidase Activities in Two genotypes of Wheat Under N A C L Salinity Stress

机译:N A C L盐度胁迫下两种基因型小麦α,β-淀粉酶和α-糖苷酶活性的变化

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Two wheat differing in salt sensitivity, was examined for osmolyte contents and activities of α-amylase, β-amylase and α-glucosidase enzymes involved in seeds germination, in absence as well as in presence of 100, 150, 200 and 300 mM NaCl. The inhibitory effects of NaCl differed, depending on the species tested. In wild wheat specie (Triticum monococcum), with reduced germination percentage and lower relative water content, the increase in NaCl concentration resulted in the decrease in endogenous level of proline, total soluble sugars and activities of the main enzymes involved in the germination process. In contrast, cultivated wheat specie (Triticum aestivum) seed in response to salt stress accumulated higher proline and total soluble carbohydrate concentrations which improved their water status and the enzyme activities involved in the germination process. Differential response of the different species of wheat to salt stress is governed by the accumulation of osmolytes in seeds.
机译:在不存在和存在100、150、200和300 mM NaCl的情况下,检查了两种对盐敏感性不同的小麦的渗透液含量以及参与种子萌发的α-淀粉酶,β-淀粉酶和α-葡萄糖苷酶的活性。 NaCl的抑制作用因所测试的物种而异。在野生小麦品种(单粒小麦)中,发芽率降低且相对含水量降低,NaCl浓度的增加导致脯氨酸的内源性水平,总可溶性糖含量降低以及发芽过程中涉及的主要酶活性降低。相反,响应盐胁迫的栽培小麦品种(Triticum aestivum)种子积累了较高的脯氨酸和总可溶性碳水化合物浓度,从而改善了它们的水分状况和发芽过程中涉及的酶活性。不同种类的小麦对盐胁迫的差异反应受渗透液在种子中的积累控制。

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