Root Growth and Water Extraction Response of Doubled-Haploid Rice Lines to Drought and Rewatering during the Vegetative Stage

摘要

Doubled haploid lines (DHLs) of rice (Oryza sativa L.) were used to examine responses to drought and rewatering in controlled rainfed lowland conditions, in order to determine whether confounding by unrelated traits would be less than has been reported previously for contrasting cultivars that differ in genetic background. IR62266 and four DHLs derived from the cross between IR62266 and CT9993 (DHL-32, -51, -54 and -79) were grown in pot experiments in the greenhouse during the 2000 dry and wet seasons at IRRI, Los Ba?os, Philippines. There were two water regimes (well-watered and drought). Estimated water extraction obtained by time domain reflectometry (TDR) was similar to cumulative transpiration estimated from pot weighing for each genotype. Genotypic variation was observed in root traits and water extraction, with extraction slower in DHL-32 and faster in DHL-79, especially in deeper soil layers. An upper bound relationship between water extraction from a soil layer and root length density (RLD) in that layer was readily apparent over DHLs and soil depths, suggesting a critical value of RLD for water extraction of 0.30 cm cm-3 in these conditions. Because soils in the field would not be as homogenous as the puddled soils used in these greenhouse experiments, this critical RLD for water extraction from a soil layer is a reference for ideal conditions, and requires careful validation in the field. Use of DHLs permitted comparisons with reduced confounding by genetic background, with consequent improvements in precision.