Transient co?￠????expression for fast and high?￠????yield production of antibodies with human?￠????like N ?￠????glycans in plants

摘要

Plant?￠????based transient expression is potentially the most rapid and cost?￠????efficient system for the production of recombinant pharmaceutical proteins, but safety concerns associated with plant?￠????specific N ?￠????glycosylation have hampered its adoption as a commercial production system. In this article, we describe an approach based on the simultaneous transient co?￠????expression of an antibody, a suppressor of silencing and a chimaeric human ???21,4?￠????galactosyltransferase targeted for optimal activity to the early secretory pathway in agroinfiltrated Nicotiana benthamiana leaves. This strategy allows fast and high?￠????yield production of antibodies with human?￠????like N ?￠????glycans and, more generally, provides solutions to many critical problems posed by the large?￠????scale production of therapeutic and vaccinal proteins, specifically yield, volume and quality.