Development of a biomedical virtual microscopy slide scanner using a line scan charge-coupled device (CCD) camera

摘要

This study developed a high speed biomedical tissue slide scanner using a line scan CCD camera to capture continuous images and complete a virtual slide of a panoramic image in real time. The virtual slide provides highly reliable data for pathology researchers in determining the pathogenic origin of biomedical tissues. The scanning system consisted of a motion control module, an image acquisition module, an image-processing module and a human-machine interface. During the image stitching process,many factors decrease the quality of the image, such aspositioning errorsand environmentaleffects. The purpose of thisstudy was to speed scanning and obtain highly reliable images of biomedical tissues. This study applied pattern matching technique to image stitching and used a color-filter to resolve problems oflack of back lighting uniformity. This study also proposes that using auto-focus methods to overcome the lack of depth of field (DOF) problems results in rapid and high-quality scans of biomedical tissues.