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Screening and characterization of Phosphorus solubilizing Bacteria and their effect on Rice seedlings

机译:增磷细菌的筛选,表征及其对水稻幼苗的影响

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An experiment was carried out to isolate, screen and characterize bacteria collected from an industrially polluted site of Bhaluka under the Mymensingh district and to evaluate their phosphorus (P) solubilizing capacity. About ten plant and soil samples from six different spots were collected from the site. Thirty four bacterial isolates were screened and pure cultures of the different bacterial isolates were prepared. Among the bacterial isolates 25 were gram negative and 9 were gram positive. About 31 bacterial isolates had catalase producing capacity and remaining 3 were negative to catalase test. Bacterial isolates were grown on a NBRIP media to determine their phosphorus solubilizing capacity. About 25 bacterial isolates were shown P solubilizing capacity. Isolate SB8 gave the highest result about 11.42 PSI (phosphorus solubilizing index), whereas other bacterial isolates showed moderate P solubilizing capacity (PSI 1.75-6.35). A plant trial with selected isolates (SB8, SB15, SB25) were also done and SB8 achieved 10% higher P content in comparison with control which supports the in vitro P solubilization assays. DOI: http://dx.doi.org/10.3329/ralf.v1i1.22353 Res. Agric., Livest. Fish. 1 (1): 27-35, Dec 2014.
机译:进行了一项实验,以分离,筛选和鉴定从Mymensingh区下的Bhaluka工业污染场所收集的细菌,并评估其磷(P)增溶能力。从该地点收集了六个不同地点的大约十种植物和土壤样品。筛选了34种细菌分离株,并制备了不同细菌分离株的纯培养物。在细菌分离物中,25克革兰氏阴性,9克革兰氏阳性。约31株细菌具有过氧化氢酶生产能力,其余3株对过氧化氢酶测试呈阴性。细菌分离株在NBRIP培养基上生长,以确定其磷增溶能力。显示出约25种细菌分离物具有P增溶能力。分离株SB8的最高结果约为11.42 PSI(磷溶解指数),而其他细菌分离株显示出中等的P溶解能力(PSI 1.75-6.35)。还完成了使用所选分离物(SB8,SB15,SB25)的工厂试验,与支持体外P增溶测定的对照相比,SB8的P含量提高了10%。 DOI:http://dx.doi.org/10.3329/ralf.v1i1.22353 Res。 Agric。,Livest。鱼。 1(1):27-35,2014年12月。

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