Eficacy of Gloriosa Superba L. On Plant Pathogenic Fungi

摘要

Gloriosa superba is a endangered toxic plant belong the family Colchicaceae (Liliaceae) commonly known as kalihari (Hindi), glory lily (English) containing high levels of colchicines and gloriocine both are toxic alkaloid. Traditionally it is used for the treatment of various diseases. The aim of present research screening of phytochemical profile and evaluate the Antifungal activity of Gloriosa superba against four plant pathogenic fungal spp. namely Fusarium oxysporum, Alternaria species, Sclerotium rolfsii and Rhizoctonia solani. These selected fungi causes a wide range of commercially significant plant disease; some time also in animals and human. To determine the antifungal activity, food poisoning method was employed. Different concentration of methanoic extract (6.25, 12.5, 25, 37.5, 50, 62.5 -g/ml) of Gloriosa superba were tested against fungal strain and the phytochemical screening were perform by standard harbone method. The methanol extract of Gloriosa superba L. showed very good antifungal activity against selected plant pathogen fungi. The excellent inhibitory activity was observed against Rhizoctonia solanii (99.33%) and Alternaria sp. (98.13%) followed by Sclerotium rolfsii (84.26%) and Fusarium oxysporum (63.23%) at the concentration of 62.5 -g/ml. The results of the phytochemical screening revealed the presence of alkaloids, steroid, carbohydrates, tannins, phenolic compounds, terpenoid, cardiac glycoside, flavonoids and absence of phlobotannin, saponin, anthraquinone. It may be due to presence of phytochemicals like colchicines (alkaloid) plant show antifungal activity. The excellent inhibitory results conclude that the crude plant extact can be used as alternative of biopesticide and some antifungal drugs.