Quinalphos Induced Oxidative Stress Biomarkers in Liver and Kidney of Common Carp, Cyprinus Carpio

摘要

Oxidative stress responses were evaluated in liver and kidney of freshwater fish, Cyprinus carpio exposed toorganophosphorus pesticide, quinalphos (25% emulsified concentration). Lipid peroxidation levels andantioxidant enzyme activities were measured spectrophotometrically in liver and kidney of fish, treated withtwo sub-lethal concentrations of quinalphos viz., 0.275 ppm and 0.55 ppm along with control as a referencefor 4, 8, 16 and 32 days. The experimental concentration of quinalphos evoked different degrees of activityin both the tissues, the liver and the kidney. The significant increase in lipid peroxidation suggests quinalphos-mediated free radical production; the increase in the activity of glutathione-S-transferase with correspondingincrease in the activity of glutathione reductase indicates the glutathione conjugation of the pesticide; activityof superoxide dismutase, catalase and glutathione peroxidase decreased drastically after 32 nd day exposureto sub-lethal concentrations of pesticide. Overall, the results demonstrate that alteration in the antioxidantenzymes, glutathione system and induction of lipid peroxidation reflects the toxicity of quinalphos which maycause oxidative stress in the experimental fish. The study, therefore, provides a rational use of biomarkers ofoxidative stress in biomonitoring programs.