Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α.
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机译：响应面法（RSM）用于优化重组大肠杆菌DH5α生产植酸酶的培养条件。植酸酶生产的最佳预测栽培条件为：种子龄3小时，接种量2.5％，L-阿拉伯糖浓度0.20％，细胞浓度0.3（在600 nm处测量）和诱导后17小时，预测的植酸酶活性为4194.45 U / mL。模型经过验证，结果显示植酸酶活性与实验值之间没有显着差异（P = 0.305）。在最佳培养条件下，重组大肠杆菌DH5α的植酸酶活性比野生型生产者阪崎肠杆菌ASUIA279的植酸酶活性高364倍。因此，使用RSM优化培养条件可积极提高重组大肠杆菌DH5α的植酸酶产量。
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