Myeloperoxidase Inactivation Affects Neutrophil Recruitment in Zebrafish Injury-Induced Model

摘要

Uncontrolled migration and excess recruitment of neutrophils can lead to persistent inflammation, tissue damageand disease. Myeloperoxidase is a remarkable target for further understanding the immune cell migration. This studytests the hypothesis that myeloperoxidase may regulate the immune cell activity and provides a new perspective onthe treatment of immune diseases by exploring the mechanism of neutrophil migration. Studies of leukocytemigration in vivo in the zebrafish model, which set a collection of advantages both mammalian and cell lines, havegained widespread attention. In this study, we used tg (coro1a: eGFP; lyz: dsred2) and tg (lyz: eGFP) lines labellingboth macrophages and neutrophils to study the effect of mpx on neutrophil chemo taxis to wounds. We found thatmyeloperoxidase was required for neutrophil migration to the wound site in injury-induced inflammation, but notrequired for neutrophil migration to the infection site in the infection-induced inflammation. Further, the regulation ofmyeloperoxidase was specific to neutrophil migration to wound inflammation, but was not necessary for macrophagemigration. Thus, myeloperoxidase activity shows therapeutic potential for inflammatory disease related to neutrophilmigration.