Isolation and Identification of Pathogenic Fungus from African Catfish (Clarias gariepinus) Eggs and Adults in National Fishery and Aquatic Life Research Center Hatchery, Ethiopia

摘要

Isolation and identification of pathogenic fungi from African Catfish (Clarias gariepinus) eggs and adults inEthiopian National Fishery and Aquatic Life Research Center hatchery was conducted from October 2015 to May2016. The aim of this study was to investigate the aquatic fungal flora associated with eggs and brood stock fromAfrican catfish in the center. A total of 16 egg samples, 77 swab samples from skin of adult fishes (brood stock) andwater samples from 14 incubating containers were investigated. Samples were collected from water, outer bodysurface of fish as well as from artificially hatched eggs. Isolation and identification of the fungus was done oncolonial and microscopic characteristics. In this study, 84.11% samples were positive for fungal growth and fungusisolates belonging to seven different genera were identified. Tricophyton, Saprolegnia, Rhizopus, Penicillium, Mucor,Microsporum and Alternaria were among the dominant isolated fungal genera. Among the isolated genera,Tricophyton was detected from 13.08% of the samples, while Alternaria had the least with 3.74%, unidentified fungiaccounts for 14.02% and unidentified yeast were 20.56%. Based on the results, various pathogenic fungi specieswere identified from fish body, water and hatched eggs, hence are potential causes of mortality and decreased egghatchability. In the light of this, appropriate egg and water disinfection methods are essential for hatcherymanagement. Fishes in rearing facilities must be given proper health management monitoring to prevent outbreak offungal disease.