SEPARATION OF SELECTED PESTICIDES BY AN HPLC TECHNIQUE; PERFORMANCE PARAMETERS AND VALIDATION

摘要

The aim of this work is to get the performance parameters investigated by hight performance liquid chromatography (HPLC), for the separation method of 2,4-Dichlorophenoxiacetic acid, 3,6-Dichloro-2-methoxybenzoic acid, an organic mixture with herbicide action. The chromatographic separation with better peak shape was achieved. The retention times (t R), peak resolutions (R S ), separation factors ( ? ), column efficiency (N eff ), height of theoretical plates (HETP), indicate that the mobile phases in gradient, containing acetonitrile and water with 1% acetic acid are the best for the separation of investigated components on cromatographic column C18. Also it has been shown that in data conditions the methode is sensitive, precise and reproductible.